ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF KITOLOD (Hippobroma longiflora) LEAVES AGAINST STREPTOCOCCUS PYOGENES

Grace Isabella Tanaja, Endang Isbandiati, Inge Wattimena

Abstract


Background: Antimicrobial resistance to antibiotics has been emerging as a serious issue in healthcare, hence the need to discover and develop new treatment alternatives. Chemical compunds found in medicinal plants can potentially be used to synthesize new antibiotic agents, one of which is the leaves of kitolod (Hippobroma longiflora). Phytochemical screening of kitolod leaves showed positive results for secondary metabolites presumed to have antibacterial effects. Objectives: The purpose of this study was to examine the antibacterial activity of ethanolic extract of kitolod leaves against Streptococcus pyogenes. Methods: Antibacterial activity of the ethanolic extract of various concentrations (0,25%, 1,75%, 3,25%, 4,75%, 6,25%) were evaluated by broth microdilution method on a 96-well microplate and by streaking on agar plates. Results: Minimium Bactericidal Concentration (MBC) of ethanolic extract of kitolod leaves against Streptococcus pyogenes was 1,75%. Minimum Inhibitory Concentration (MIC) of ethanolic extract of kitolod leaves against Streptococcus pyogenes could not be inferred. Conclusion: Ethanolic extract of kitolod leaves showed antibacterial activities against Streptococcus pyogenes.

Keywords


Antibacterial; Kitolod; Hippobroma longiflora; Streptococcus pyogenes

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References


Sims Sanyahumbi A, Colquhoun S, Wyber R, Carapetis JR. Global disease burden of group a streptococcus.

Carroll KC, Butel JS, Morse SA, Mietzner T. Jawetz, melnick & adelbergs medical microbiology 27e. McGraw-Hill Education; 2016. 867p.

Vekemans J, Gouvea-Reis F, Kim JH, Excler JL, Smeesters PR, O'Brien KL, et al. The path to group a streptococcus vaccines: world health organization research and development technology roadmap and preferred product characteristics. Clin Infect Dis. 2019;69(5):87783.

Ferretti JJ, Stevens DL, Fischetti V a. Streptococcus pyogenes: Basic biology to clinical manifestations. streptococcus pyogenes Basic Biol to

Clin Manifestations [Internet]. 2016 [cited 2020 May 4];115. Available

from:http://www.ncbi.nlm.nih.gov/books/NBK333418/pdf/Bookshelf_NBK33341 8.pdf%5Cnhttp://www.ncbi.nlm.nih.gov/pubmed/26866208

Brook I. Treatment challenges of group a beta-hemolytic streptococcal pharyngotonsillitis. Int Arch Otorhinolaryngol. 2017;21(3):28696.

Kanwal S, Vaitla P. Streptococcus Pyogenes [Internet]. StatPearls.

StatPearls Publishing; 2020 [cited 2020 May 11]. Available from:

http://www.ncbi.nlm.nih.gov/pubmed/32119415

Passli D, Lauriello M, Passli GC, Passli FM, Bellussi L. Group A

streptococcus and its antibiotic resistance. Acta Otorhinolaryngol Ital.

;27(1):2732.

Abachi S, Lee S, Rupasinghe HPV. Molecular mechanisms of inhibition of streptococcus species by phytochemicals. Vol. 21, Molecules.

131 p.

Simanjutak H. Antibacterial activity of ethanolic extract of kitolod

(hippobroma longiflora) leaf against staphylococcus aureus and salmonella typhi. Asian J Pharm Res Dev. 2020;8(1):524.

Resting JR, Tolderlund IL, Pedersen AF, Witt M, Jaroszewski JW, Staerk D. Piperidine and tetrahydropyridine alkaloids from Lobelia siphilitica and Hippobroma longiflora. J Nat Prod. 2009;72(2):3125.

Ibrahim S, Restia Rezki D. Isolation and elucidation structure of

triterpenoids from hippobroma longiflora leaf extract and tested of

antibacterial activity. Available online www.jocpr.com J Chem Pharm Res

[Internet]. 2017;2017(11):2058. Available from: www.jocpr.com

Fazil M, Suci RN, Allfiah F, Alam DN, Angelia G, Situmeang B, et al. Analisis senyawa alkaloid dan flavonoid dari ekstrak kitolod ( isotoma longiflora ) dan uji aktivitasnya terhadap bakteri penyebab karies gigi. Itekimia. 2017;2(1):7383.

Arfiandi P, Ramdhani I, Makhfirra UI, Riza Marjoni M. Growth inhibition test of Streptococcus mutans against methanol extract/fractions of Kitolod (Isotoma longiflora (L.) C. Int J Res

Pharm Pharm Sci 16 Int J Res Pharm Pharm Sci [Internet]. 2018;3(6):168. Available from: www.pharmacy journal.in

Klan?nik A, Piskernik S, Jerek B, Moina SS. Evaluat ion of diffusion and dilution methods to determine the antibacterial activity of plant extracts. JMicrobiol Methods. 2010;81(2):1216.




DOI: https://doi.org/10.33508/jwmj.v4i1.3578

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