POLYMORPHISM OF INTERLEUKIN-10 (-819) GENE IS NOT ASSOCIATED WITH LIVER DAMAGE AMONG CHRONIC HEPATITIS B PATIENTS

Abstract

Introduction: Chronic hepatitis B (CHB) is characterized by chronic liver inflammation and
hepatocellular damage caused by hepatitis B virus (HBV), leading to fatal liver diseases suchas cirrhosis and hepatocellular carcinoma (HCC). Liver damage causes elevated levels ofserum alanine aminotransferase (ALT). Previous studies have shown that IL-10 genepolymorphism (-819 C/T) is linked to several diseases, including inflammatory, autoimmunediseases, and cancer.
Purpose: To investigate the relationship between IL-10 gene polymorphisms (-819 C/T) andliver damages in chronic hepatitis B patients at Arifin Achmad Regional Hospital, Riau
Province.
Method: This study was an analytical study with a cross-sectional approach. Liver damageswere examined by measuring ALT levels, and IL-10 gene polymorphisms were analyzedusing Amplification-Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR).
Results: There were 74 subjects included in this study, aged 45.4713.42 years old,
consisting of 43 males (58.1%) and 31 females (41.9%). Of 74 subjects, 35 CHB patients
were presented without complication, 26 CHB patients were complicated with cirrhosis, and13 CHB patients were complicated with hepatoma. The genotype of the IL-10 (-819) genepolymorphism in this study was found as follows: CT genotype in 34 subjects (45.9%), TTgenotype in 30 subjects (40.5%), and CC genotype in 10 subjects (13.5%). There was no
significant relationship between IL-10 gene polymorphisms (- 819) and liver damage in
chronic hepatitis B patients (p> 0.05).
Conclusion: IL-10 gene polymorphism (-819) may not contribute to liver damages among
CHB patients.