Ence Of ESBL Bacteria In Baby Box Handle At Dr. Soetomo Hospital

Manik Retno Wahyunitisari, Martono Tri Utomo, Nicholas Fernando Purnomo


Health Associated Infection (HAI) in neonates can increase neonatal infection risk, which is a fairly frequent cause of neonatal death. Microorganisms that are quite often found to contaminate include gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae. Gram-negative bacteria are also quite often found to have resistance to antibiotic therapy that is usually given, especially actlactam, and will increase the degree of disease to mortality, this bacterium is called ESBL. The study was conducted to determine the level of ESBL bacterial contamination in health facilities at Dr. Soetomo general hospital. Swabs are taken and biochemical tests were done to identify pathogen species. These isolates were also tested for ESBL production by the double-disc synergy test (DDST). There were 30 samples that contaminate the handle of the baby box, 2 of which (6.67%) tested positive for ESBL. With good hand hygiene, the use of disinfectants in medical devices, floors, walls, and doors, maintenance and replacement of tap water filters, and regular monitoring can reduce the number of bacterial contamination.

Save to Mendeley


Extended Spectrum  lactamase, baby box handle, Dr. Soetomo General Hospital; Health Associated Infection

Full Text:



Xu, J., Murphy, S., Kochanek, K., Bastian, B. and Arias, E. (2019). Deaths: Final Data for 2016. National Vital Statistics Reports, 67(5), p.2.

Anggraini, D., Sholihin, U., Savira, M., Djojosugito, F., Irawan, D. and Rustam, R. (2018). Prevalensi dan Pola Sensitivitas Enterobacteriaceae Penghasil ESBL di RSUD Arifin Achmad Pekanbaru. Jurnal Kedokteran Brawijaya, 30(1), p.49.

Engda, T., Moges, F., Gelaw, A., Eshete, S. and Mekonnen, F. (2018). Prevalence and antimicrobial susceptibility patterns of extended spectrum beta-lactamase producing Entrobacteriaceae in the University of Gondar Referral Hospital environments, northwest Ethiopia. BMC Research Notes, [online] 11(1). Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964971/ [Accessed 14 Apr. 2019].

Tenenbaum, T., Becker, K., Lange, B., Martin, A., Schäfer, P., Weichert, S. and Schroten, H. (2016). Prevalence of Multidrug-Resistant Organisms in Hospitalized Pediatric Refugees in an University Children’s Hospital in Germany 2015-2016. Infection Control & Hospital Epidemiology, 37(11), p.1311-1312.

Cdc.gov. (2019). Laboratory Detection of Extended-Spectrum β-Lactamases (ESBLs) | HAI | CDC. [online] Available at: https://www.cdc.gov/hai/settings/lab/lab_esbl.html [Accessed 15 Apr. 2019].

Shaikh, S., Fatima, J., Shakil, S., Rizvi, S. and Kamal, M. (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences, [online] 22(1), pp.90-101. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4281622/ [Accessed 29 May 2019].

Roberts, T., Limmathurotsakul, D., Turner, P., Day, N., Vandepitte, W. and Cooper, B., 2019. Antimicrobial-resistant Gram-negative colonization in infants from a neonatal intensive care unit in Thailand. Journal of Hospital Infection, 103(2), pp.151-155.

Wanger, A., Chavez, V., Huang, R., Wahed, A., Actor, J. and Dasgupta, A., 2017. Microbiology And Molecular Diagnosis In Pathology. 1st ed. ELSEVIER, pp.51-60.

Tille, P., 2017. Bailey & Scott's Diagnostic Microbiology. 14th ed. St. Louis, Missouri: ELSEVIER, pp.330-354.

Cdc.gov. 2010. Klebsiella Pneumoniae In Healthcare Settings | HAI | CDC. [online] Available at: [Accessed 17 June 2020].

Baharutan, A., Rares, F. and Soeliongan, S., 2015. Pola bakteri penyebab infeksi nosokomial pada ruang perawatan intensif anak di BLU RSUP Prof DR. R. D. Kandou Menado Jurnal e-Biomedik, 3(1), p.414.

Cannon, C. and Linnemann, C., 1992. Yersinia enterocolitica Infections in Hospitalized Patients: The Problem of Hospital-Acquired Infections. Infection Control and Hospital Epidemiology, 13(3), pp.139-143.

Cdc.gov. 2016. Information For Health & Lab Professionals | Yersinia | CDC. [online] Available at: [Accessed 17 June 2020]

DOI: https://doi.org/10.33508/jwm.v6i2.2789