Uji Aktivitas Antibakteri dan Antibiofilm Fraksi Biji Kelengkeng (Euphoria longan Lour. Steud.) terhadap Staphylococcus aureus ATCC 6538

Oda Shantina Prasety, Lisa Soegianto, Sumi Wijaya

Abstract


Longan (Euphoria longan Lour. Steud.) has an antibacterial potential. Longan seed is one part of Longan that has antibacterial activity. This study was conducted to determine the antibacterial and antibiofilm activity of longan seed fraction (Euphoria longan Lour. Steud.) against Staphylococcus aureus ATCC 6538. In this study, the extract was obtained from maceration using 96% ethanol and it was fractionated by liquid-liquid solvent method using nhexane, ethyl acetate, and water. Each fraction prepared at concentration 100000 ppm, and then the antibacterial activity on Staphylococcus aureus was determined by well diffusion method, antibiofilm activity using microdilution method, and phytochemical screening to determine the metabolite compounds that have
antibacterial and antibiofilm activity using thin layer chromatography. The results showed that the diameter of growth inhibition in ethyl acetate fraction with a concentration of 100000 ppm was 14.59 mm 0.01, n-hexane fraction at 7.15 mm 0.63 and water fraction of 12.52 mm 0.40. Antibiofilm test results showed that the percentage of growth inhibition of biofilm on the fraction of ethyl acetate with a concentration of 100000 ppm was 99.08%. Phytochemical screening using thin layer chromatography carried out on the fraction of ethyl acetate and showed that the fraction contains tannin/polyphenols, triterpenoid saponins and flavonoids.

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References


Archer, N.K., Powers, M.E., Leid, J.G., Costerton, J.W., Mazaitis, M.J., and Shirtliff, M.E. 2011. Staphylococcus aureus Biofilm: Properties, Regulation and Roles in Human Disease, Landes Bioscience, 2(5): 445-459.

Boulton A. J, Krisner S., Vileykite L.,2004, Neurophatic diabetic foot ulcers,N Engl J Med, 351(1): 48 55.

Brand PW. The foot in diabetes.in Rifkin H,Raskin P ; Diabetes mellitus, vol v, American Diabetes Association, Prentice-Hall International, Maryland 2000, 291-5.

Cushnie, T. P. T. and Lamb, A. J. 2005. Antimicrobial Activity of Flavonoids, International Journal of Antimicrobial Agents, 26(5): 343-356.

Drecoli, E., Karimi, J., Manaf, A., Syahbuddin, S. 2007. Artikel Penelitian: Profil Ulkus Diabetik pada Penderita Rawat Inap di Bagian Penyakit Dalam RSUP. Dr. M. Djamil Padang. (Akses: 11 Februari 2016, 23:40).

Haris et al. 2002. An Introduction to Staphylococcus aureus, and Techniques for Identifying and Quantifying S. aureus Adhesins in Relation to Adhesion to Biomaterials Review. European Cells and Materials Vol. 4.

John L., Mark E., Roger G. 2006. Biofilms, Infection, and Antimicrobial Therapy. CRC Press Taylor & Francis Group, New York.

Kementrian Negara Riset dan Teknologi. 2006. Euphoria longana Lamk., WARINTEK. Diakses pada 21 Juni 2016, http://warintek.ristekdikti.go.id/pangan_kesehatan/tanamanobat/depkes/4-037.pdf.

Melchoir, MB, H Vaarkamp and J Fink-Gremmels, 2006. Bio

fillm: Arole in recurrent mastitis infection ? The Vet. Journal. 171: 398407.

Monalisa, D., Handayani, T., Sukmawati, D., 2011, Uji Daya Antibakteri Ekstrak daun Tapak Liman (Elephantopus scaber L.) Terhadap Staphylococcus aureus dan Salmonella typhi, Jurnal BIOMA. 9(2): 13 20.

Naim, R. 2014, Senyawa Antimikroba Dari Tumbuhan, Fakultas Kedokteran Hewan Dan Sekolah Pascasarjana Institut Pertanian Bogor.

Paraje, M. G. et al. 2011. Antimicrobial resistance in biofilms. Argentina: Formatex.

Santi, R. N., Muhtadi, Indrayudha, P. 2010. Aktivitas Antibakteri Ekstrak Etanol Biji dan Kulit Lengkeng (Euphoria longan Lour. Steud.) terhadap Escherichia coli dan Staphylococcus aureus serta Toksisitasnya terhadap Artemia salina Leach. Jurnal Farmasi Indonesia Pharmacon (Pharmaceutical Journal of Indonesia). Surakarta: Fakultas Farmasi Muhammadiyah Surakarta.

Soong, Y. Y. dan Barlow, P. J. 2005. Isolation and Structure Elucidation of Phenolic Compounds from longan (Dimocarpus longan Lour.) seed by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry, Journal of Chromatography A,1085(2): 270-277. PMid:16106708.http://dx.doi.org/10.1016/j. chroma.2005.06.042

Tjokroprawiro, A. 2007. Buku ajar ilmu penyakit dalam. Airlangga University Press, Surabaya.

Waspaji, S. 2006. Buku Ajar Ilmu Penyakit Dalam Edisi IV. Jakarta: Pusat Penerbitan IPD FKUI.




DOI: https://doi.org/10.33508/jfst.v6i2.2235