Karakterisasi dan Uji Aktivitas Antibakteri dari Fungi Endofit Ranting Tanaman Manggis (Garcinia mangostana L.) terhadap Escherichia coli dan Staphylococcus aureus

Kevin Widjaja, Lisa Soegianto, Martha Ervina

Abstract


Resistance is commonly caused by improper usage of antibiotics, so the treatment of the infection always needs a new and more potent compounds. Endophytic microbes are microbes that all or part of its life stay in the tissues of plant, which established a mutually beneficial relationship between them. In this research, isolation of endophytic fungi was conducted by sticking the sterilized twigs of mangosteen to the media Malt Extract Agar obtained three pure endophytic fungi colonies. The antibacterial activity assay with direct inoculation method at media Plate Count Agar which was inoculated with bacteria and showed that the antibacterial activity against Escherichia coli ATCC 8739 was one isolate (ER2) with inhibition average ratio 1.42 and against Staphylococcus aureus ATCC 6538 was three isolates (ER1, ER2, and ER3) with inhibition average ratio 1.66; 1.37; and 1.89. The fungus were observed characteristics of the macroscopic, microscopic and biochemical assay. ER1 and ER3 isolates are suspected as a Penicillium genus with specific characteristics such as the conidiophore, fialid, metulae, and conidiospore. ER2 isolate was suspected as a Rhizoctonia genus because there are nearly right-angled hyphae and monilioid cells.

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References


Barnett HL, Hunter, and Sarry, B, 1972, Illustrated Genera of Imperfect Fungi, 3rd ed., Burgess Publishing Company, Minnesota.

Cole RJ and Schweikert MA, 2003, Handbook of Secondary Fungal Metabolites, Academic Press Elsevier Science, California.

Elfina D, Martina A dan Roza RM, 2014, Isolasi dan Karakterisasi Fungi Endofit dari Kulit Buah Manggis (Garcinia mangostana L.) sebagai Antimikroba terhadap Candida albicans, Staphylococcus aureus dan Escherichia coli, Skripsi, Kampus Binawidya Pekanbaru, Pekanbaru.

Hadioetomo RS, 1993, Mikrobiologi Dasar dalam Praktek, PT. Gramedia, Jakarta.

Kumala S, Dwi HJ, dan Priyo W, 2008, Isolasi Mikroba Endofit Ranting Tumbuhan Trengguli (Cassia fistula L.) dan Aktivitas Enzim Xilanase, Jurnal Bahan Alam Indonesia, 6(4):1412-2855.

Kumala S, 2014, Mikroba Endofit, PT. ISFI Penerbitan, Jakarta.

Morton JF, 1987, Fruits of Warm Climates, diakses pada tanggal 18 November 2015, http://www.hort.purdue.edu/newcrop/morton/mangosteen.html

Murray RK, Bender DA, Botham KM, Kennelly PJ, dan Rodwell VW, 2014, Biokimia Harper, edisi 29, Penerbit Buku Kedokteran EGC, Jakarta.

Purwanto UMS, Pasaribu FH, dan Bintang M, 2014, Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri, Current Biochemistry Journal. 1(1): 51-57.

Strobel G, Daisy B, and Castillo U, 2004, Natural Products from Endophytic Microorganisms, Journal of Natural Products, 67(2):257-268.

Suwandi U, 1992, Mekanisme Kerja Antibiotik, PT. Kalbe Farma, Jakarta.

Van Steenis CGGJ, 2008, Flora, Diterjemahkan dari Bahasa Belanda oleh Moeso Surjowinoto, PT Pradnya Paramita, Jakarta.

Watanabe T, 2010, Pictorial Atlas of Soil and Seed Fungi, Morphologies and Cultured Fungi and Key to Species, 3rd ed., CRC Press, USA.




DOI: https://doi.org/10.33508/jfst.v6i1.2007