THE DIFFERENCES IN RAT CHOLESTEROL LEVELS AFTER GIVING RED GUAVA JUICE

Ni MDS Hayati, Dewa AL Dewi, Chrisdina P Sari

Abstract


Introduction: Cholesterol is a fat found in our body, it can be dangerous if cholesterol level is increasing. The way to lower cholesterol levels, such as by consuming high-fiber foods for example red guava.

Purpose: To determine the difference in total cholesterol levels in rats after giving red guava juice.

Method: The research was conducted using 30 Rattus Norvegicus. Rats were divided into three groups, each group consisted of 10 rats. Control group was given 594 chicken feed, first treatment group was given lard and second treatment group was given lard with red guava juice. Before being given treatment, five rats from each group were examined for their total cholesterol and the remaining five rats were given treatment for 14 days before total cholesterol were checked. Examination of total cholesterol by enzymatic colorimetry CHOD-PAP using blood from orbital vein of rats without fasting.

Result: There were significant differences in total cholesterol of rats before and after treatment (P<0.05) in each treatment group. In comparison of three groups that had given treatment, significant difference was found (P=0.005). Significant differences were also found in comparison of two groups, between first treatment group and second treatment group (P = 0.004), insignificant results were found in comparison of control group with first treatment group (P = 0.060), and comparison of control group with second treatment group. (P=0.299).

Conclusion: There are differences in total cholesterol of rats, total cholesterol of rats in high-fat diet group with guava juice were lower than the high-fat diet group.


Keywords


Red Guava; Total Cholesterol

Full Text:

PDF

References


Widada ST, Martsiningsik M atik, Carolina stephanie cicilia. Gambaran perbedaan kadar kolesterol total CHOD-PAP (Cholesterol Oxidase-Peroxidase Aminoanypirin) sampel serum dan sampel plasma EDTA. J Teknol Lab. 2016;5(1):1214.

Widiartha F, Sartika RAD. Gula darah sebagai faktor dominan hiperkolesterolemia. Dep Gizi Kesehat Masy Univ Indones. 2020;6(27):2530.

Kemenkes.RI. Profil penyakit tidak menular tahun 2016. Vol. 53, Journal of Chemical Information and Modeling. 2016. 400 p.

Tijjani H, Banbilbwa Joel E, Luka CD. Modulatory effects of some Fruit juices on lipid profile in rats fed with high lipid diet. Asian J Biochem Genet Mol Biol. 2020;3(2):18.

Rahman muhammad aulia, Hamdani I, Thrisity I, Chalil muhammad jalauddin assuyuthi. Perbandingan efektivitas jus buah tomat (lycopersicum esculantum mill.) dengan juas buah jambu biji merah (psidium guajava L.) terhadap penurunan kadar kolesterol total pada tikus jantan yang diinduksi kuning telur. J Ilm simantek. 2019;3(3):168999.

Nugraheni AA, Jaelani M, Rahayuni A, Semedi P. Efektifitas pemberian jus tomat dan jambu biji merah terhadap penurunan kolesterol total pada wanita overweight. J Ris Gizi. 2019;7(2):1204.

Maryanto S, Fatimah S, Marsono Y. The effect of red guava fruits supplementation on the SCFA and cholesterol production in. Agritech. 2013;33(3):3349.

Ardian J, Jauhari MT, Rahmiati BF. Pengaruh pemberian jus jambu biji merah terhadap penurunan kadar LDL dan kolesterol total. Nutr J. 2020;1(1):2634.

Maryanto S, Marsono Y. The effect of guava on the improvement of lipid profile in hypercholesterolemic rats. IOP Conf Ser Earth Environ Sci. 2019;276(1).

Mulyani NS, Al Rahmad AH, Jannah R. Faktor resiko kadar kolesterol darah pada pasien rawat jalan penderita jantung koroner di RSUD Meuraxa. Aceh Nutr J. 2018;3(2):132.

Harini M, Astirin OP. Kadar kolesterol darah tikus putih (Rattus norvegicus ) hiperkolesterolemik setelah perlakuan VCO. Nusant Biosci. 2019;1:538.

Sena NAP, Sapang M, Palupi KC. Hubungan asupan zat gizi, indeks massa tubuh, dan aktivitas fisik terhadap kadar kolesterol darah total pada pasien penyakit jantung koroner Di RSUD Kota Prabumulih. J Kesehat Masy. 2020;1:112.

Rosyida R, Yuniarti Y, Mintarsih SN, Ambarwati R, Larasati MD. Efektifitas pemberian jus jambu biji terhadap kadar kolesterol total pada pralansia overweight. J Ris Gizi. 2018;6(2):31.

Sartika RAD. Pengaruh asam lemak jenuh, tidak jenuh dan asam lemak trans terhadap kesehatan. Kesmas Natl Public Heal J. 2008;2(4):154.




DOI: https://doi.org/10.33508/jwmj.v4i4.4339

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Creative Commons License
Journal of Widya Medika Junior is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License