THE EFFECTIVENESS OF HAEMATOCOCCUS PLUVIALIS EXTRACT ON SUPEROXIDE DISMUATSE LEVEL OF RATTUS NORVEGICUS POST ULTRAVIOLET B EXPOSURE

Angeline G Irwan, Endang Isbandiati, Dave G Oenarta

Abstract


Background : Exposure of ultraviolet B from the sun can trigger the formation of free radicals, and causing oxidative damage on skin. One of the treatments for skin damage caused by UV B exposure is using 2% glutathione cream. and using green algae Haematococcus pluvialis extract cream.

Objective : To analize the differences in the effectiveness of giving Haematococcus pluvialis extract cream and glutathione cream on white rats post ultraviolet B exposure.

Method : The type of research used in this study is experimental with the post test only control group design approach. The number of samples was calculated using the Federer formula, with a total sample of 30 white rats that are devided into 6 groups. The parameter is superoxide dismutase enzim that is measured using spectrophotometer.

Result : As a normal control group, 5 white rats were not exposed to UV B and were not given any cream while 25 white rats were exposed to UV B light for 2 hours in 14 days, then 5 rats as negative control group were not given any cream, 5 rats as positive control group were given 2% glutathione cream, 5 rats were given 10% Haematococcus pluvialis cream, 5 rats were given 20% Haematococcus pluvialis cream, and 5 rats were given 30% Haematococcus pluvialis cream. The results of this research showed that rats that were given 20% and 30% Haematococcus pluvialis cream has higher superoxide dismutase levels than the other groups.

Conclusion : Haematococcus pluvialis extract cream is effective in treating oxidating damage on white rats post ultraviolet B exposure.


Keywords


Haematoccus plucialis estract cream; glutathione cream; superoxide dismutase; ultraviolet B.

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DOI: https://doi.org/10.33508/jwmj.v4i4.4337

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